EasyPGX ready BRAF -Model RT022 -Real Time PCR Kit
Detection of the main mutations of codon 600 of the gene BRAF using 4 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each sample.
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Controls
The kit is provided with the following controls:
- Positive control sample containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA;
- Negative control.
Sensitivity
The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAMb and HEX. LOD down to 0.5%.
Starting material
The kit allows the analysis of DNA extracted from fresh, frozen, formalin-fixed paraffin-embedded (FFPE) tissues and plasma*.