Easy EGFR - Model RT003 - Real Time PCR Kit
From Easy Line - Real Time PCR Kits
Main features Detection of the main mutations of exons 18, 19, 20, 21 of EGFR gene using 8 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each sample.
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Controls Control DNA positive for all the mutation...
Controls Control DNA positive for all the mutations detected by the kit. Reference standard DNA Horizon EGFR E746-A750 1% to monitor the analytical process and the performances of the system. The standard is characterized by a well defined allelic ratio wild-type/mutant.
Sensitivity The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAM and HEX (LOD down to 0.5%).
Starting material The kit allows the analysis of DNA extracted from fresh, frozen and formalin-fixed paraffin-embedded tissue.
Execution time 2 hours.
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