AmoyDx - Novel Coronavirus (2019-nCoV) Detection Kit
From Real-time PCR Assays
The novel coronaviruses belong to the p genus. COVID-19 is an acute respiratory infectious disease. People are generally susceptible. Currently, the patients infected by the novel coronavirus are the main source of infection; asymptomatic infected people can also be an infectious source. Based on the current epidemiological investigation, the incubation period is 1 to 14 days, mostly 3 to 7 days. The main manifestations include fever, fatigue and dry cough. Nasal congestion, runny nose, sore throat, myalgia and diarrhea are found in a few cases.
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Details
Intended Use
The AmoyDx Novel Coronavirus (2019-nCoV) Detection Kit is a real-time reverse transcription (RT)-PCR assay for qualitative detection of novel coronavirus SARS-CoV-2. The kit is intended use with viral RNA extracted from human upper and lower respiratory specimens.
The kit is intended to be used by trained professionals in a laboratory environment.
Principles of the Procedure
The kit is based on real-time RT-PCR technology, combines reverse transcription and PCR amplification in one-step procedure. It's designed for a specific amplification of the ORF lab (open reading frame, ORF lab) and N (Nucleoprotein, N) conserved regions of novel coronavirus SARS-CoV-2 in viral RNA. The targeted region is amplified by several specific primers and detected by fluorescence probes. A non-competitive internal control is included in nCoV RNA detection system to assess RNA quality and monitor the whole PCR procedure.
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